This lab course is designed to help students become conversant in the language and application of modern genetics and science in general. The goal of this course-based undergraduate research experience (CURE) is to help you to (1) learn genetics lab skills, (2) use scientific practices, (3) generate new understandings of the natural world, (4) contribute to a real research project, (5) develop your collaborative research skills, and (6) learn how iteration (i.e. repetition) is an important part of the research process. I will assess how much you learn over the course of the semester with practicals and at the beginning and end of the semester. After successfully completing this course, most students should be able to complete several specific learning objectives.
Upon successful completion of this course, you should know / understand:
· How precision and accuracy influence the results of genetics lab techniques (SUU ELO 11.0)
· You should be able to: Properly use a micropipette; Make buffer solutions in the correct concentrations
· Assessed with: Notebook entries, Lab practicals
· How the physical characteristics of DNA and RNA cause them to be extracted differently (SUU ELO 11.0)
· You should be able to: Extract DNA from biological samples
· Assessed with: Notebook entries, Lab practicals
· How to visualize DNA on agarose gels (SUU ELO 11.0)
· You should be able to: Load an agarose gel and use electrophoresis to move DNA through the gel
· Assessed with: Notebook entries, Lab practicals
· How Polymerase Chain Reaction (PCR) works to amplify DNA (SUU ELO 11.0)
· You should be able to: Set up a PCR and measure amplified DNA
· Assessed with: Notebook entries, Lab practicals
· How DNA sequencing occurs and what it means. (SUU ELO 11.0)
· You should be able to: Interpret sequencing data from an external lab facility
· Assessed with: Notebook entries, Lab practicals
· How DNA barcodes are used to identify organisms and answer research questions. (SUU ELO 11.0)
· You should be able to: Upload your organism’s DNA sequence to BOLD or BLAST then taxonomically identify it.
· Assessed with: Notebook entries Research poster, Class presentation
· How scientific practices are used in a genetics lab. (SUU ELO 15.0)
· You should be able to: Ask questions related to problems that can be solved with genetic techniques; propose hypotheses; select appropriate genetic methods; gather and analyze genetic data; interpret messy real world data; and present data to an audience of your peers or non-peers.
· Assessed with: Class presentation, Research poster, Teamwork assignments
· How to effectively engage in a scientific collaboration to develop your skills in working with a group to complete a genetics-based research project. (SUU ELO 15.0)
· You should be able to: Contribute to team meetings, facilitate the contributions of team members, fulfill your individual contributions outside of team meetings, and foster a constructive team climate to further the collaborative goals of your team.
· Assessed with: Teamwork assignments
· How the process of scientific discovery is applied in genetics. (SUU ELO 4.0)
· You should be able to: Identify when your genetic results are sufficiently convincing to draw conclusions and be worthy of contribution to the larger research database for this project.
· Assessed with: Notebook entries, Teamwork assignments, Research poster
· How scientists use pilot data to build their research programs. (SUU ELO 4.0)
· You should be able to: Create a convincing figure or table.
· Assessed with: Research poster